The main intent of the proposed Conference was to enable the major members of the scientific community working with different microbial biological control agents (fungi, bacteria, viruses) having various backgrounds (molecular biology, formulation, genetics, risk assessment, biochemistry, synthetic biology, ecology, physiology, agronomy, nanotechnology, natural products chemistry) to meet and interact also with scientists working on the same types of microorganisms but with different purposes. Breaking the impasse to the adoption of microbial bioherbicides is needed more than ever, as agriculture is losing synthetic herbicides because of their banning and loss of utility because of rapidly evolving resistance. This multi-disciplinary group, including university, government, and industry representatives, gave lectures covering the latest and most advanced research, followed by long periods of discussions. The group discussed how the newest ideas, approaches, solutions, or methodologies coming from different fields of application may solve the biological, technical, and regulatory issues that have limited the success of microbial bioherbicides compared to other microbial biopesticides. Moreover, discussions with stakeholders gave new insights into regulatory aspects and technology transfer.